2009年03月12日

STRUCTURL BIOLOGY : Molecular mecanichnery in action

If biology were a car, structural biologists would be looking under the bonnet to find out how the engine works.

Put more prosaically, structural biology aims to understand how biology works at the molecular level.

Much information is gleaned by studying at atomic resolution the three-dimensional structurs of molecules that make up living organisms, and the interactions of these molecules with one another.


### DataBace ###
nature Vol.445 567-682 Issue no.7128 8 Febuary 2007
News and Views p.609 / STRUCTURL BIOLOGY : Molecular mecanichnery in action / Ad Bax and Dennis A. Torchia
Articles p.618 / Quantitative dynamics and binding studies of the 20S proteasome by NMR / R Sprangers & L E Kay (The Universuty of Tronto)


The machinery used by the cell to perform essential biological processes is made up of large molecukar assemblies. One such complex, the proteasome, is the central molecular machine for removal of damaged and misfolded protenis from the cell.

Here we show that for the 670-kilodalton 20S proteasome core particle it is possible to overcome the molecular weight limitations that have tradionally hampered quantitative nuclear magnetic resonance (NMR) spectroscopy studies of such large systems.

This is a achieved by using an istope labelling scheme where isoleucine, and valine methyls are protonated in an otherwise highly deuterted back ground in concert with experiments that preserve the ligetimes of the resulting NMR signals.

The methodology has been applied to the 20S core particle to ewvel functionally important motions and interactions by recording spectra on complexes with molecular weihts of up to a megadalton.

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