2009年03月25日

telomerase RNA

Telomeres cap the ends of chromosomes and provide a means to complete replication. The DNA portion of telomeres is synthesized by the enzyme telomerase using part of an RNA subunit as a tenplate for reverse transcription. How the mature 3' end of telomerase RNA is generated has so far remained elusive.

Here we show that in Schizosaccharomyces pombe telomerase RNA transcriots must be rocessed to generate functional telomerase. Charecterization of the maturation pathway uncovered un unexpected role for the spliceosome, which normally catalyses splicing of pre-messenger RNA.

The first spliceosomal cleavage reaction generates the mature 3' end of telomerase RNA (TER1, the functional RNA encoded by the ter1^+ gene), releasing the active from of the RNA without exon ligation.

Blocking the first step or permitting completion of spllicing generates inactive forms of TER1 and causes progressive telomere shortening.

We establish that 3'end processing of TER1 is critical for telomerase function and describe a previously unknown mechanism for RNA maturation that uses the ability of the spliceosome to mediate site-specific cleavage.


### DataBace ###
nature Vol.456 837-1008 no.7224
Spliceosome meets tekimerase / Sophie Bonnal & Juan Valacarcel
Spliceosomal clavage generates the 3' end of telomerase RNA / J A Box et al. (Stowers Institure for Medical Research)

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